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验证数据展示

  • WB analysis of HeLa using 14914-1-AP (same clone as 14914-1-PBS)

    WB analysis of HeLa using 14914-1-AP (same clone as 14914-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)

    IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with JPT1/HN1 antibody included
  • IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)

    IHC staining of human ovary tumor using 14914-1-AP (same clone as 14914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with JPT1/HN1 antibody included
  • IP experiment of MCF-7 using 14914-1-AP (same clone as 14914-1-PBS)

    IP experiment of MCF-7 using 14914-1-AP (same clone as 14914-1-PBS)

    IP result of anti-HN1 (IP:14914-1-AP, 4ug; Detection:14914-1-AP 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 14914-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HN1 Polyclonal antibody, PBS Only

HN1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14914-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy JPT1/HN1 Ready-To-Use IHC Kit. JPT1/HN1 primary antibody included.

产品信息

14914-1-PBS targets HN1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6712

Product name: Recombinant human HN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-154 aa of BC001420

Sequence: MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPTEQPVRKNKMASNIFGTPEENQASWAKSAGAKSSGGREDLESSGLQRRNSSEASSGDFLDLKGEGDIHENVDTDLPGSLGQSEEKPVPAAPVPSPVAPAPVPSRRNPPGGKSSLVLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hematological and neurological expressed 1
别名
计算分子量 11 kDa, 16 kDa, 19 kDa
观测分子量 23-30 kDa
GenBank蛋白编号BC001420
基因名称 HN1
Gene ID (NCBI) 51155
RRIDAB_10863118
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UK76
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth.

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