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HMHA1 Polyclonal antibody

HMHA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14832-1-AP

Print datasheet

Synonyms

HA 1, HLA HA1, HMHA1, KIAA0223, mHag HA 1

验证数据展示

  • WB analysis using 14832-1-AP

    WB analysis using 14832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14832-1-AP (HMHA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsil using 14832-1-AP

    IHC staining of human tonsil using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human tonsil using 14832-1-AP

    IHC staining of human tonsil using 14832-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsil using 14832-1-AP (HMHA1 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inA375 cells, K-562 cells, mouse thymus tissue, HeLa cells, Jurkat cells
Positive IHC detected inhuman tonsil tissue, human spleen tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

14832-1-AP targets HMHA1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HMHA1 fusion protein Ag6636 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histocompatibility (minor) HA-1
Synonyms HA 1, HLA HA1, HMHA1, KIAA0223, mHag HA 1
Calculated Molecular Weight 1136 aa, 125 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC065223
Gene Symbol HMHA1
Gene ID (NCBI) 23526
RRIDAB_10642145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92619
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HMHA1 antibody 14832-1-APDownload protocol
IHC protocol for HMHA1 antibody 14832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Aging Dis

Microglia Exhibit Distinct Heterogeneity Rather than M1/M2 Polarization within the Early Stage of Acute Ischemic Stroke

Authors - Hongyu Ma
View Article