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验证数据展示

  • WB analysis of HeLa using 24821-1-AP

    WB analysis of HeLa using 24821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24821-1-AP (HMGXB4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24821-1-AP

    IP experiment of HeLa using 24821-1-AP

    IP result of anti-HMGXB4 (IP:24821-1-AP, 4ug; Detection:24821-1-AP 1:500) with HeLa cells lysate 2560ug.

HMGXB4 Polyclonal antibody

HMGXB4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24821-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

HMG box containing protein 4, HMG box domain containing 4, HMG2L1, HMGBCG, HMGXB4, Protein HMGBCG, THC211630

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24821-1-AP targets HMGXB4 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 HMGXB4 fusion protein Ag20424 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HMG box domain containing 4
别名 HMG box containing protein 4, HMG box domain containing 4, HMG2L1, HMGBCG, HMGXB4, Protein HMGBCG, THC211630
计算分子量 601 aa, 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC104754
基因名称 HMGXB4
Gene ID (NCBI) 10042
RRIDAB_2879742
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGXB4 also termed as HMG box containing protein 4 or HMG2L1 is a 601 amino acid protein, which contains one HMG box DNA-binding domain. HMGXB4 negatively regulates Wnt/beta-catenin signaling during development. HMG-2L1 is expressed in the nucleus and may play a role in transcriptional regulation.

实验方案

Product Specific Protocols
WB protocol for HMGXB4 antibody 24821-1-APDownload protocol
IP protocol for HMGXB4 antibody 24821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols