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验证数据展示

  • WB analysis using 10953-1-AP (same clone as 10953-1-PBS)

    WB analysis using 10953-1-AP (same clone as 10953-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10953-1-AP (HMGN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10953-1-AP (same clone as 10953-1-PBS)

    IHC staining of human prostate cancer using 10953-1-AP (same clone as 10953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10953-1-AP (HMGN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10953-1-AP (same clone as 10953-1-PBS)

    IF Staining of HepG2 using 10953-1-AP (same clone as 10953-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGN2 antibody (10953-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10953-1-PBS in a different storage buffer formulation.

HMGN2 Polyclonal antibody, PBS Only

HMGN2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10953-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

High mobility group nucleosome-binding domain-containing protein 2, HMG17, Non-histone chromosomal protein HMG-17

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10953-1-PBS targets HMGN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1401

Product name: Recombinant human HMGN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of BC014644

Sequence: MPKRKAEGDAKGDKAKVKDEPQRRSARLSAKPAPPKPEPKPKKAPAKKGEKVPKGKKGKADAGKEGNNPAENGDAKTDQAQKAEGAGDAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 high-mobility group nucleosomal binding domain 2
别名 High mobility group nucleosome-binding domain-containing protein 2, HMG17, Non-histone chromosomal protein HMG-17
计算分子量 9 aa, 3 kDa
观测分子量 18-20 kDa
GenBank蛋白编号BC014644
基因名称 HMGN2
Gene ID (NCBI) 3151
RRIDAB_2118482
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05204
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HMGN2 is a highly conserved nucleosomal protein which may involve in unfolding higher-order chromatin structure and facilitating the transcriptional activation of mammalian genes. It is the most abundant, ubiquitous and evolutionarily conserved nonhistione proteins found in the nuclei of higher eukaryotes. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer.

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