验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A549 cells, HepG2 cells, LNCaP cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17643-1-AP targets HMGCS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HMGCS1 fusion protein Ag11825 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble) |
| 别名 | Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMGCS, HMG-CoA synthase, HMG CoA synthase, EC:2.3.3.10 |
| 计算分子量 | 520 aa, 57 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC083514 |
| 基因名称 | HMGCS1 |
| Gene ID (NCBI) | 3157 |
| RRID | AB_2248359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01581 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| IHC protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| IF protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| IP protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis
| ||
Nat Cancer Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism
| ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
J Cell Biol TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination. | ||
Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. | ||
Mol Cell Proteomics Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression. |