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验证数据展示

  • WB analysis using 83482-4-RR (same clone as 83482-4-PBS)

    WB analysis using 83482-4-RR (same clone as 83482-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83482-4-RR (HMGB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83482-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83482-4-RR (same clone as 83482-4-PBS)

    FC experiment of HeLa using 83482-4-RR (same clone as 83482-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGB2 Recombinant antibody (83482-4-RR, Clone:240453C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83482-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83482-4-RR

    Affinity and Kinetic Characterization of 83482-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83482-4-RR against Human HMGB2 were performed. The affinity constant is 16.4 nM.

HMGB2 Recombinant monoclonal antibody, PBS Only

HMGB2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83482-4-PBS

产品说明书

CloneNo. 240453C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HMGB2 Ready-To-Use IHC Kit. HMGB2 primary antibody included.

产品信息

83482-4-PBS targets HMGB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6135

Product name: Recombinant human HMGB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 44-207 aa of BC001063

Sequence: KCSERWKTMSAKEKSKFEDMAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPSAFFLFCSEHRPKIKSEHPGLSIGDTAKKLGEMWSEQSAKDKQPYEQKAAKLKEKYEKDIAAYRAKGKSEAGKKGPGRPTGSKKKNEPEDEEEEEEEEDEDEEEEDED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 high-mobility group box 2
别名 HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2
计算分子量 24 kDa
GenBank蛋白编号BC001063
基因名称 HMGB2
Gene ID (NCBI) 3148
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP26583
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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