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验证数据展示

  • WB analysis using 82973-1-RR (same clone as 82973-1-PBS)

    WB analysis using 82973-1-RR (same clone as 82973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82973-1-RR (HMGB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82973-1-PBS

    IF Staining of HeLa using 82973-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMGB1 antibody (82973-1-PBS, Clone: 230182G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 82973-1-PBS

    FC experiment of HeLa using 82973-1-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGB1 Recombinant monoclonal antibody, PBS Only (82973-1-PBS, Clone:230182G6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis of HeLa using 82973-1-RR (same clone as 82973-1-PBS)

    WB analysis of HeLa using 82973-1-RR (same clone as 82973-1-PBS)

    WB result of HMGB1 antibody (82973-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with negative control and HMGB1 knockout HeLa cells. This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82973-1-RR (same clone as 82973-1-PBS)

    IHC staining of human colon using 82973-1-RR (same clone as 82973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82973-1-RR (HMGB1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HMGB1 antibody included
  • IHC staining of human colon using 82973-1-RR (same clone as 82973-1-PBS)

    IHC staining of human colon using 82973-1-RR (same clone as 82973-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82973-1-RR (HMGB1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HMGB1 antibody included
  • FC experiment of HeLa using 82973-1-RR (same clone as 82973-1-PBS)

    FC experiment of HeLa using 82973-1-RR (same clone as 82973-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HMGB1 Recombinant antibody (82973-1-RR, Clone:230182G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82973-1-RR (same clone as 82973-1-PBS)

    IF Staining of HeLa using 82973-1-RR (same clone as 82973-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMGB1 antibody (82973-1-RR, Clone: 230182G6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82973-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82973-1-PBS

    Affinity and Kinetic Characterization of 82973-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82973-1-PBS against Human HMGB1 were performed. The affinity constant is 0.246 nM.

  • Affinity and Kinetic Characterization of 82973-1-RR

    Affinity and Kinetic Characterization of 82973-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82973-1-RR against Human HMGB1 were performed. The affinity constant is 0.246 nM.

  • Featured Product
  • KD/KO Validated

HMGB1 Recombinant monoclonal antibody, PBS Only

HMGB1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82973-1-PBS

产品说明书

CloneNo. 230182G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

230182G6, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HMGB1 Ready-To-Use IHC Kit. HMGB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82973-1-PBS targets HMGB1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1264

Product name: Recombinant human HMGB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-215 aa of BC003378

Sequence: MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 high-mobility group box 1
别名 230182G6, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1
计算分子量 25 kDa
观测分子量25-30 kDa
GenBank蛋白编号BC003378
基因名称 HMGB1
Gene ID (NCBI) 3146
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09429
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).

实验方案

Product Specific Protocols
FC protocol for HMGB1 antibody 82973-1-PBSDownload protocol
IF protocol for HMGB1 antibody 82973-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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