×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 20795-1-AP (same clone as 20795-1-PBS)

    WB analysis using 20795-1-AP (same clone as 20795-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 20795-1-AP (same clone as 20795-1-PBS)

    IHC staining of human pancreas cancer using 20795-1-AP (same clone as 20795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HMGA2 antibody included
  • IF Staining of A549 using 20795-1-AP (same clone as 20795-1-PBS)

    IF Staining of A549 using 20795-1-AP (same clone as 20795-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using HMGA2 antibody (20795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 20795-1-AP (same clone as 20795-1-PBS)

    IP experiment of NIH/3T3 using 20795-1-AP (same clone as 20795-1-PBS)

    IP result of anti-HMGA2 (IP:20795-1-AP, 4ug; Detection:20795-1-AP 1:1000) with NIH/3T3 cells lysate 3200ug. This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 20795-1-AP (same clone as 20795-1-PBS)

    IHC staining of human pancreas cancer using 20795-1-AP (same clone as 20795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HMGA2 antibody included
  • Featured Product
  • KD/KO Validated

HMGA2 Polyclonal antibody, PBS Only

HMGA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20795-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

High mobility group AT-hook protein 2, High mobility group protein HMGI-C, HMGI C, HMGIC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HMGA2 Ready-To-Use IHC Kit. HMGA2 primary antibody included.

产品信息

20795-1-PBS targets HMGA2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14588

Product name: Recombinant human HMGA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-109 aa of NM_003483

Sequence: MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWPQQVVQKKPAQEETEETSSQESAEED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 high mobility group AT-hook 2
别名 High mobility group AT-hook protein 2, High mobility group protein HMGI-C, HMGI C, HMGIC
计算分子量 108 aa, 12 kDa
观测分子量 18-20 kDa
GenBank蛋白编号NM_003483
基因名称 HMGA2
Gene ID (NCBI) 8091
RRIDAB_2665377
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52926
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (PMID: 18202751,19551524). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (18425117). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (19551524). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (PMID: 20228781). The calcualted molecular weight of HMGA2 is 12 kDa, but modified HMGA2 is about 18-20 kDa. (PMID: 18505920 )

||
New chat

Able™

正在加载，请稍候...