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验证数据展示

  • WB analysis using 67354-1-Ig

    WB analysis using 67354-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67354-1-Ig (HMG20B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 67354-1-Ig

    IHC staining of human skin cancer using 67354-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HMG20B Monoclonal antibody

HMG20B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67354-1-Ig

产品说明书

CloneNo. 4C2A9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

4C2A9, BRAF25, BRAF35, BRCA2 associated factor 35, BRCA2-associated factor 35

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67354-1-Ig targets HMG20B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6103

Product name: Recombinant human HMG20B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 73-284 aa of BC002552

Sequence: PVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTLDFYM

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 high-mobility group 20B
别名 4C2A9, BRAF25, BRAF35, BRCA2 associated factor 35, BRCA2-associated factor 35
计算分子量 36 kDa
观测分子量 40 kDa
GenBank蛋白编号BC002552
基因名称 HMG20B
Gene ID (NCBI) 10362
RRIDAB_2882609
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9P0W2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation (PMID:10449787). The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse (PMID:15240883). HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure (PMID:19997604). The predicted molecular weight of the HMG20B protein is 36 kDa, while the molecular weight detected in the literature is approximately 40 kDa by SDS-PAGE (PMID: 36171271).

实验方案

Product Specific Protocols
WB protocol for HMG20B antibody 67354-1-IgDownload protocol
IHC protocol for HMG20B antibody 67354-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols