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HMG20B Monoclonal antibody

HMG20B Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C2A9

Cat No : 67354-1-Ig

Print datasheet

Synonyms

BRAF25, BRAF35, BRCA2 associated factor 35, FLJ26127, high mobility group 20B, HMG box containing protein 20B, HMG20B, HMGX2, HMGXB2, PP7706, pp8857, SMARCE1 related protein, SMARCE1r, SOXL

验证数据展示

  • WB analysis using 67354-1-Ig

    WB analysis using 67354-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67354-1-Ig (HMG20B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 67354-1-Ig

    IHC staining of human skin cancer using 67354-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67354-1-Ig (HMG20B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67354-1-Ig targets HMG20B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen HMG20B fusion protein Ag6103 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name high-mobility group 20B
Synonyms BRAF25, BRAF35, BRCA2 associated factor 35, FLJ26127, high mobility group 20B, HMG box containing protein 20B, HMG20B, HMGX2, HMGXB2, PP7706, pp8857, SMARCE1 related protein, SMARCE1r, SOXL
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC002552
Gene Symbol HMG20B
Gene ID (NCBI) 10362
RRIDAB_2882609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P0W2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].

实验方案

Product Specific Protocols
WB protocol for HMG20B antibody 67354-1-IgDownload protocol
IHC protocol for HMG20B antibody 67354-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols