验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
| Positive IP detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32178-1-AP targets HMCN2 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37696 Product name: Recombinant human HMCN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 330-536 aa of XM_011518465.3 Sequence: TLEWPLQGVPISLVINSTGLKAPGRLDSVELAQSSGKPLLTLPTKPLSNGSTHQLWGGPPFHTPKERFYLKVKGKDHEGNPLLRVSGVSYSGVAPGAPLVSMAPRIHGYLHQPLLVSCSVHSALPFRLQLRRGEARLGEERHFQESGNSSWEILRASKAEEGTYECTAVSRAGTGRAKAQIVVTDPPPQLVPAPNVTVSPGETAVLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hemicentin 2 |
| 别名 | Hemicentin 2 , Hemicentin-2 |
| 计算分子量 | 542kDa，5059aa |
| 观测分子量 | 550 kDa |
| GenBank蛋白编号 | XM_011518465.3 |
| 基因名称 | HMCN2 |
| Gene ID (NCBI) | 256158 |
| RRID | AB_3742536 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8NDA2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HMCN2 (Hemicentin-2) is a large extracellular matrix protein belonging to the fibronectin family. HMCN2 proteins form a complex network of microfibrils in the extracellular matrix that is essential for the maintenance of tissue structure and function. HMCN2 is expressed in epithelial cells and muscle tissues, and may play an important role in muscle-tendon attachment. HMCN2 expression and localization suggests that it has an important role in maintaining the integrity of skin and muscle tissue. It may maintain tissue structure and function by interacting with other extracellular matrix proteins, such as fibronectin and elastic fibers.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for HMCN2 antibody 32178-1-AP | Download protocol |
| WB protocol for HMCN2 antibody 32178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
