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验证数据展示

  • IHC staining of human colon using 18837-1-AP

    IHC staining of human colon using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HMCN1 antibody included
  • IHC staining of human colon using 18837-1-AP

    IHC staining of human colon using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HMCN1 antibody included
  • WB analysis of mouse skin using 18837-1-AP

    WB analysis of mouse skin using 18837-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 18837-1-AP (HMCN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 18837-1-AP

    IF Staining of hTERT-RPE1 using 18837-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse lung using 18837-1-AP

    IHC staining of mouse lung using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18837-1-AP (HMCN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMCN1 antibody included
  • IHC staining of human appendicitis using 18837-1-AP

    IHC staining of human appendicitis using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 18837-1-AP (HMCN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMCN1 antibody included
  • IHC staining of human stomach cancer using 18837-1-AP

    IHC staining of human stomach cancer using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18837-1-AP (HMCN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMCN1 antibody included
  • IHC staining of mouse liver using 18837-1-AP

    IHC staining of mouse liver using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18837-1-AP (HMCN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMCN1 antibody included
  • IHC staining of rat lung using 18837-1-AP

    IHC staining of rat lung using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 18837-1-AP (HMCN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMCN1 antibody included
  • IHC staining of rat stomach using 18837-1-AP

    IHC staining of rat stomach using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 18837-1-AP (HMCN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMCN1 antibody included
  • IHC staining of rat stomach using 18837-1-AP

    IHC staining of rat stomach using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 18837-1-AP (HMCN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HMCN1 antibody included

HMCN1 Polyclonal antibody

HMCN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18837-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

ARMD1, FBLN6, FIBL 6, FIBL6, FIBL-6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman colon tissue, human stomach cancer tissue, human appendicitis tissue, rat lung tissue, rat stomach tissue, mouse liver tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy HMCN1 Ready-To-Use IHC Kit. HMCN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18837-1-AP targets HMCN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemicentin 1
别名 ARMD1, FBLN6, FIBL 6, FIBL6, FIBL-6
计算分子量 613 kDa, 600 kDa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号NM_031935
基因名称 HMCN1
Gene ID (NCBI) 83872
RRIDAB_2878563
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RW7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-976 amino acids of human HMCN1 can recognize all three isoforms of HMCN1.

实验方案

Product Specific Protocols
IF protocol for HMCN1 antibody 18837-1-APDownload protocol
IHC protocol for HMCN1 antibody 18837-1-APDownload protocol
WB protocol for HMCN1 antibody 18837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Exp Med

HMCN1 variants aggravate epidermolysis bullosa simplex phenotype

Authors - Shir Bergson
View Article
ratWB

Front Pharmacol

Artemisinin relieves myocardial ischemia-reperfusion injury via modulating miR-29b-3p and hemicentin 1

Authors - Junyu Han
View Article
mouseIHC

Front Cell Dev Biol

Hemicentin-1 is an essential extracellular matrix component during tooth root formation by promoting mesenchymal cells differentiation

Authors - Yujia Cui
View Article
mouseIHC

PLoS Pathog

Reduction of antisense transcription affects bovine leukemia virus replication and oncogenesis

Authors - Thomas Joris
View Article
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