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验证数据展示

  • WB analysis using 67456-1-Ig

    WB analysis using 67456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67456-1-Ig

    IHC staining of human breast cancer using 67456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67456-1-Ig

    IHC staining of human breast cancer using 67456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HMBS Monoclonal antibody

HMBS Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67456-1-Ig

产品说明书

CloneNo. 1G12H3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, HepG2 cells, Caco-2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67456-1-Ig targets HMBS in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag6594

Product name: Recombinant human HMBS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-361 aa of BC000520

Sequence: MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hydroxymethylbilane synthase
别名 HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS
计算分子量 39 kDa
观测分子量 39-42 kDa
GenBank蛋白编号BC000520
基因名称 HMBS
Gene ID (NCBI) 3145
RRIDAB_2882688
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08397
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for HMBS antibody 67456-1-IgDownload protocol
WB protocol for HMBS antibody 67456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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