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验证数据展示

  • WB analysis using 14797-1-AP

    WB analysis using 14797-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14797-1-AP

    IF Staining of MCF-7 using 14797-1-AP

    Immunofluorescent analysis of MCF-7 cells, using HMBS antibody 14797-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HEK-293 using 14797-1-AP

    IP experiment of HEK-293 using 14797-1-AP

    IP result of anti-HMBS (IP:14797-1-AP, 4ug; Detection:14797-1-AP 1:2000) with HEK-293 cells lysate 1200 ug.

HMBS Polyclonal antibody

HMBS Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 14797-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

EC:2.5.1.61, hydroxymethylbilane synthase, PBG D, PBGD, PBG-D

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, A375 cells, Raji cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inMCF-7 cells, Hela cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14797-1-AP targets HMBS in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6509

Product name: Recombinant human HMBS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-361 aa of BC000520

Sequence: MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxymethylbilane synthase
别名 EC:2.5.1.61, hydroxymethylbilane synthase, PBG D, PBGD, PBG-D
计算分子量 39 kDa
观测分子量 39-42 kDa
GenBank蛋白编号BC000520
基因名称 HMBS
Gene ID (NCBI) 3145
RRIDAB_2117447
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08397
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for HMBS antibody 14797-1-APDownload protocol
IP protocol for HMBS antibody 14797-1-APDownload protocol
WB protocol for HMBS antibody 14797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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