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验证数据展示

  • WB analysis of HEK-293 using 14046-1-AP

    WB analysis of HEK-293 using 14046-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14046-1-AP (HLCS antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 14046-1-AP

    IHC staining of human ovary cancer using 14046-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14046-1-AP (HLCS antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 14046-1-AP

    IF Staining of HEK-293 using 14046-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HLCS antibody (14046-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HLCS Polyclonal antibody

HLCS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14046-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Biotin protein ligase, Biotin apo-protein ligase, Biotin--[acetyl-CoA-carboxylase] ligase, Biotin--[methylcrotonoyl-CoA-carboxylase] ligase, Biotin--[methylmalonyl-CoA-carboxytransferase] ligase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, human kidney tissue, human liver tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14046-1-AP targets HLCS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5208

Product name: Recombinant human HLCS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 425-726 aa of BC060787

Sequence: SYVSEVEITPSCIPVVTNMEAFSSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQDINLRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQHKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQVHLGSAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPKRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
别名 Biotin protein ligase, Biotin apo-protein ligase, Biotin--[acetyl-CoA-carboxylase] ligase, Biotin--[methylcrotonoyl-CoA-carboxylase] ligase, Biotin--[methylmalonyl-CoA-carboxytransferase] ligase
计算分子量 81 kDa
观测分子量 76 kDa
GenBank蛋白编号BC060787
基因名称 HLCS
Gene ID (NCBI) 3141
RRIDAB_2117166
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50747
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for HLCS antibody 14046-1-APDownload protocol
IHC protocol for HLCS antibody 14046-1-APDownload protocol
WB protocol for HLCS antibody 14046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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