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验证数据展示

  • WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HLA-G antibody included
  • IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HLA-G antibody included

HLA-G Monoclonal antibody, PBS Only

HLA-G Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66447-1-PBS

产品说明书

CloneNo. 1E5A10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HLA-G Ready-To-Use IHC Kit. HLA-G primary antibody included.

产品信息

66447-1-PBS targets HLA-G in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10839

Product name: Recombinant human HLA-G protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-308 aa of BC021708

Sequence: SHSMRYFSAAVSRPSRGEPRFIAMGYVDDTQFVRFDSDSACPRMEPRAPWVEREGPEYWEEETRNTKAHAQTDRMNLQTLRGYYNQSEASSHTLQWMIGCDLGSDGRLLRGYEQYAYDGKDYLALNEDLRSWTAADTAAQISKRKCEAANVAEQRRAYLEGTCVEWLHRYLENGKEMLQRADPPKTHVTHHPVFDYEATLRCWALGFYPAEIILTWQRDGEDQTQDVELVETKPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLMLRWKQSSLPTIPI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 major histocompatibility complex, class I, G
别名 HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G
计算分子量 338 aa, 38 kDa
观测分子量 33-45 kDa
GenBank蛋白编号BC021708
基因名称 HLA-G
Gene ID (NCBI) 3135
RRIDAB_2881816
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17693
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). HLA-G is a non-classical MHC class I molecule with multiple immunoregulatory properties. HLA-G exhibits a restricted pattern of expression that includes placental extravillous trophoblasts at the maternal-fetal interface, where it abolishes maternal immune cell activity against fetus and establishes immune tolerance. Aberrant expression of HLA-G has been found in a variety of human neoplastic diseases. It plays an important role in the escape of tumor cells from immunosurveillance.