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验证数据展示

  • WB analysis using 66819-1-Ig

    WB analysis using 66819-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66819-1-Ig (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66819-1-Ig

    IHC staining of human tonsillitis using 66819-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66819-1-Ig

    IHC staining of human tonsillitis using 66819-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of Raji using 66819-1-Ig

    IP experiment of Raji using 66819-1-Ig

    IP result of anti-HLA-F (IP:66819-1-Ig, 4ug; Detection: 66819-1-Ig 1:2000) with Raji cells. Lysate: 1mg. 25% of IP elution was used for Western blot detection.

  • IF Staining of Raji using 66819-1-Ig

    IF Staining of Raji using 66819-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-F antibody (66819-1-Ig, Clone: 1B12C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

HLA-F Monoclonal antibody

HLA-F Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66819-1-Ig

产品说明书

CloneNo. 1B12C2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

1B12C2, CDA12, HLA 5.4, HLA CDA12, HLA F

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, NCCIT cells, Raji cells, human peripheral blood leukocytes
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66819-1-Ig targets HLA-F in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6475

Product name: Recombinant human HLA-F protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-298 aa of BC062991

Sequence: AGSHSLRYFSTAVSRPGRGEPRYIAVEYVDDTQFLRFDSDAAIPRMEPREPWVEQEGPQYWEWTTGYAKANAQTDRVALRNLLRRYNQSEAGSHTLQGMNGCDMGPDGRLLRGYHQHAYDGKDYISLNEDLRSWTAADTVAQITQRFYEAEEYAEEFRTYLEGECLELLRRYLENGKETLQRADPPKAHVAHHPISDHEATLRCWALGFYPAEITLTWQRDGEEQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPQPLILRWEQS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 major histocompatibility complex, class I, F
别名 1B12C2, CDA12, HLA 5.4, HLA CDA12, HLA F
计算分子量 39 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC062991
基因名称 HLA-F
Gene ID (NCBI) 3134
RRIDAB_2882162
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP30511
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

实验方案

Product Specific Protocols
IF protocol for HLA-F antibody 66819-1-IgDownload protocol
IHC protocol for HLA-F antibody 66819-1-IgDownload protocol
WB protocol for HLA-F antibody 66819-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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