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验证数据展示

  • WB analysis using 15733-1-AP

    WB analysis using 15733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15733-1-AP (HLA-DRB4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 15733-1-AP

    IHC staining of human tonsillitis using 15733-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15733-1-AP (HLA-DRB4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 15733-1-AP

    IHC staining of human tonsillitis using 15733-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15733-1-AP (HLA-DRB4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 15733-1-AP

    IF Staining of mouse spleen using 15733-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HLA-DRB4 antibody (15733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HLA-DRB4 Polyclonal antibody

HLA-DRB4 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 15733-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

HLA DR4B, HLA DRB4, MHC class II antigen DRB4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15733-1-AP targets HLA-DRB4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8492

Product name: Recombinant human HLA-DRB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC005312

Sequence: MVCLKLPGGSCMAALTVTLTVLSSPLALAGDTQPRFLEQAKCECHFLNGTERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSMMSPLTVQWSARSESAQSKMLSGVGGFVLGLLFLGTGLFIYFRNQKGHSGLQPTGLLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major histocompatibility complex, class II, DR beta 4
别名 HLA DR4B, HLA DRB4, MHC class II antigen DRB4
计算分子量 266 aa, 30 kDa
观测分子量 35 kDa
GenBank蛋白编号BC005312
基因名称 HLA-DRB4
Gene ID (NCBI) 3126
RRIDAB_1939612
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13762
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for HLA-DRB4 antibody 15733-1-APDownload protocol
IHC protocol for HLA-DRB4 antibody 15733-1-APDownload protocol
WB protocol for HLA-DRB4 antibody 15733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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