验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Daudi cells, Ramos cells, pig lung tissue |
| Positive IHC detected in | human tonsillitis tissue, rat skin tissue, human stomach cancer tissue, mouse lung tissue, mouse skin tissue, human rectal cancer tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Raji cells |
Planning an IHC experiment? We recommend our IHCeasy HLA-DRA Ready-To-Use IHC Kit. HLA-DRA primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
68543-1-Ig targets HLA-DRA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, pig |
| 免疫原 |
CatNo: Ag11224 Product name: Recombinant human HLA-DRA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 24-218 aa of BC032350 Sequence: WAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class II, DR alpha |
| 别名 | 3D6E8, HLA DRA, HLA DRA1, HLA DRB, HLA-DR |
| 计算分子量 | 254 aa, 29 kDa |
| 观测分子量 | 32-34 kDa |
| GenBank蛋白编号 | BC032350 |
| 基因名称 | HLA-DRA |
| Gene ID (NCBI) | 3122 |
| ENSEMBL Gene ID | ENSG00000204287 |
| RRID | AB_3085250 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P01903 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HLA-DRA antibody 68543-1-Ig | Download protocol |
| IHC protocol for HLA-DRA antibody 68543-1-Ig | Download protocol |
| WB protocol for HLA-DRA antibody 68543-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
