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HLA-DPB1 Polyclonal antibody

HLA-DPB1 Polyclonal Antibody for FC, IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

FC, IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11298-1-AP

Print datasheet

Synonyms

DPB1, HLA DP1B, HLA DPB1, HLA-DPB1, MHC class II antigen DPB1

验证数据展示

  • WB analysis using 11298-1-AP

    WB analysis using 11298-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 11298-1-AP

    IHC staining of human tonsillitis using 11298-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 11298-1-AP

    IHC staining of human tonsillitis using 11298-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11298-1-AP (HLA-DPB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 11298-1-AP

    IF Staining of human tonsillitis using 11298-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HLA-DPB1 antibody (11298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human tonsillitis using 11298-1-AP

    IF Staining of human tonsillitis using 11298-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HLA-DPB1 antibody (11298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Daudi using 11298-1-AP

    FC experiment of Daudi using 11298-1-AP

    1X10^6 Daudi cells were intracellularly stained with 0.4 ug Anti-Human HLA-DPB1 (11298-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inDaudi cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

11298-1-AP targets HLA-DPB1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF-P, IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityrat
Immunogen HLA-DPB1 fusion protein Ag1815 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name major histocompatibility complex, class II, DP beta 1
Synonyms DPB1, HLA DP1B, HLA DPB1, HLA-DPB1, MHC class II antigen DPB1
Calculated Molecular Weight 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC015000
Gene Symbol HLA-DPB1
Gene ID (NCBI) 3115
RRIDAB_2877755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04440
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HLA-DPB1 antibody 11298-1-APDownload protocol
IHC protocol for HLA-DPB1 antibody 11298-1-APDownload protocol
IF protocol for HLA-DPB1 antibody 11298-1-APDownload protocol
FC protocol for HLA-DPB1 antibody 11298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Sci Rep

Histological and contractile changes in the genioglossus muscle after nasal obstruction in growing rats

Authors - Karin Harumi Uchima Koecklin
View Article