验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 3 publications below |
产品信息
16109-1-AP targets HLA-DPA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9031 Product name: Recombinant human HLA-DPA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-224 aa of BC009956 Sequence: IKADHVSTYAAFVQTHRPTGEFMFEFDEDEQFYVDLDKKETVWHLEEFGRAFSFEAQGGLANIAILNNNLNTLIQRSNHTQAANDPPEVTVFPKEPVELGQPNTLICHIDRFFPPVLNVTWLCNGEPVTEGVAESLFLPRTDYSFHKFHYLTFVPSAEDVYDCRVEHWGLDQPLLKHWEAQEPIQMPETTETV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class II, DP alpha 1 |
| 别名 | DP(W3), DP(W4), HLA DP1A, HLA DPA1, HLA SB alpha chain, HLADP, HLADPA1, HLA-DPA1, HLASB, MHC class II DP3 alpha, MHC class II DPA1 |
| 计算分子量 | 260 aa, 29 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC009956 |
| 基因名称 | HLA-DPA1 |
| Gene ID (NCBI) | 3113 |
| RRID | AB_1851387 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20036 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for HLA-DPA1 antibody 16109-1-AP | Download protocol |
| IHC protocol for HLA-DPA1 antibody 16109-1-AP | Download protocol |
| WB protocol for HLA-DPA1 antibody 16109-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Activation of GABA receptor attenuates intestinal inflammation by modulating enteric glial cells function through inhibiting NF-κB pathway | ||
Aging (Albany NY) HLA-DPA1 overexpression inhibits cancer progression, reduces resistance to cisplatin, and correlates with increased immune infiltration in lung adenocarcinoma | ||
iScience Single-cell transcriptomics reveals the role of antigen presentation in liver metastatic breast cancer | ||
Ocul Surf Spatial transcriptomics and single-cell RNA-sequencing revealed dendritic cell-mediated inflammation in keratoconus | ||
Front Immunol Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics |
