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验证数据展示

  • WB analysis using 82899-2-RR (same clone as 82899-2-PBS)

    WB analysis using 82899-2-RR (same clone as 82899-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82899-2-RR (HLA-DMA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82899-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82899-2-RR (same clone as 82899-2-PBS)

    IHC staining of human colon using 82899-2-RR (same clone as 82899-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82899-2-RR (HLA-DMA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82899-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82899-2-RR (same clone as 82899-2-PBS)

    IHC staining of human colon using 82899-2-RR (same clone as 82899-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82899-2-RR (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82899-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82899-2-RR

    Affinity and Kinetic Characterization of 82899-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82899-2-RR against Human HLA-DMA were performed. The affinity constant is 0.303 nM.

HLA-DMA Recombinant monoclonal antibody, PBS Only

HLA-DMA Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82899-2-PBS

产品说明书

CloneNo. 230155E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

230155E7, D6S222E, DMA, DMA protein, DMA protein MHC class II antigen Major histocompatibility complex, class II, DM alpha cDNA FLJ75740, highly similar to Homo sapiens major histocompatibility complex, class II, DM alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82899-2-PBS targets HLA-DMA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2452

Product name: Recombinant human HLA-DMA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-261 aa of BC026279

Sequence: LPHSWAVPEAPTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFGKPNTLVCFVSNLFPPMLTVNWQHHSVPVEGFGPTFVSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDRYTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGIIVGIVLIIYFRKPCSGD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 major histocompatibility complex, class II, DM alpha
别名 230155E7, D6S222E, DMA, DMA protein, DMA protein MHC class II antigen Major histocompatibility complex, class II, DM alpha cDNA FLJ75740, highly similar to Homo sapiens major histocompatibility complex, class II, DM alpha
计算分子量 261 aa, 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC026279
基因名称 HLA-DMA
Gene ID (NCBI) 3108
RRIDAB_3670628
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ31604
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.

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