- Featured Product
- KD/KO Validated
Hexokinase 1 Polyclonal antibody
Hexokinase 1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, HepG2 cells, mouse brain tissue, rat brain tissue, THP-1 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19662-1-AP targets Hexokinase 1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, pig |
Immunogen | Hexokinase 1 fusion protein Ag8015 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | hexokinase 1 |
Synonyms | Brain form hexokinase, Cell and organelle markers, Hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1 |
Calculated Molecular Weight | 102 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC008730 |
Gene Symbol | HK1 |
Gene ID (NCBI) | 3098 |
RRID | AB_10859778 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P19367 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Hexokinase 1 antibody 19662-1-AP | Download protocol |
IHC protocol for Hexokinase 1 antibody 19662-1-AP | Download protocol |
IP protocol for Hexokinase 1 antibody 19662-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Immunol NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity. | ||
Sci Adv Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease. | ||
Nat Commun SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis. | ||
Cancer Lett CYLD deficiency enhances metabolic reprogramming and tumor progression in nasopharyngeal carcinoma via PFKFB3. | ||
PLoS Biol Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex. |