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验证数据展示

  • WB analysis using 68995-1-Ig (same clone as 68995-1-PBS)

    WB analysis using 68995-1-Ig (same clone as 68995-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68995-1-Ig (HJURP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68995-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 68995-1-Ig (same clone as 68995-1-PBS)

    IF Staining of A549 using 68995-1-Ig (same clone as 68995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using HJURP antibody (68995-1-Ig, Clone: 1F8C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68995-1-PBS in a different storage buffer formulation.

HJURP Monoclonal antibody, PBS Only

HJURP Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68995-1-PBS

产品说明书

CloneNo. 1F8C5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

1F8C5, FAKTS, Fetal liver-expressing gene 1 protein, FLEG1, hFLEG1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68995-1-PBS targets HJURP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34571

Product name: Recombinant human HJURP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 394-748 aa of BC001940

Sequence: ATYNLDEENRFRTLKWLISPVKIVSRPTIRQGHGENRQREIEIRFDQLHREYCLSPRNQPRRMCLPDSWAMNMYRGGPASPGGLQGLETRRLSLPSSKAKAKSLSEAFENLGKRSLEAGRCLPKSDSSSSLPKTNPTHSATRPQQTSDLHVQGNSSGIFRKSVSPSKTLSVPDKEVPGHGRNRYDEIKEEFDKLHQKYCLKSPGQMTVPLCIGVSTDKASMEVRYQTEGFLGKLNPDPHFQGFQKLPSSPLGCRKSLLGSTAIEAPSSTCVARAITRDGTRDHQFPAKRPRLSEPQGSGRQGNSLGASDGVDNTVRPGDQGSSSQPNSEERGENTSYRMEEKSDFMLEKLETKSV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Holliday junction recognition protein
别名 1F8C5, FAKTS, Fetal liver-expressing gene 1 protein, FLEG1, hFLEG1
计算分子量 86 kDa
观测分子量85-90 kDa
GenBank蛋白编号BC001940
基因名称 HJURP
Gene ID (NCBI) 55355
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8NCD3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

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