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验证数据展示

  • WB analysis of L02 using 15302-1-AP

    WB analysis of L02 using 15302-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human liver cancer using 15302-1-AP

    IHC staining of human liver cancer using 15302-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:50 (under 40x lens).

HIST2H2AA4 Polyclonal antibody

HIST2H2AA4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15302-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

H2A/R, H2AFO, HIST2H2AA, HIST2H2AA3, HIST2H2AA4, histone, histone cluster 2, H2aa4, Histone H2A type 2 A, Histone H2A.2, Histone H2A/o

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, Jurkat cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15302-1-AP targets HIST2H2AA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag7560

Product name: Recombinant human HIST2H2AA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-130 aa of BC001629

Sequence: MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 2, H2aa4
别名 H2A/R, H2AFO, HIST2H2AA, HIST2H2AA3, HIST2H2AA4, histone, histone cluster 2, H2aa4, Histone H2A type 2 A, Histone H2A.2, Histone H2A/o
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC001629
基因名称 HIST2H2AA4
Gene ID (NCBI) 723790
RRIDAB_2878124
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6FI13
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIST2H2AA4 antibody 15302-1-APDownload protocol
IHC protocol for HIST2H2AA4 antibody 15302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Physiol Biochem

Effect of Type I Diabetes on the Proteome of Mouse Oocytes.

Authors - Guangjian Jiang
View Article