HIST1H2AC Polyclonal antibody

HIST1H2AC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15953-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

H2AC6, H2A/l, Histone H2A, histone cluster 1, H2ac, H2AFL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIST1H2AC Ready-To-Use IHC Kit. HIST1H2AC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15953-1-AP targets HIST1H2AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag8663

Product name: Recombinant human HIST1H2AC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-130 aa of BC017379

Sequence: MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 1, H2ac
别名 H2AC6, H2A/l, Histone H2A, histone cluster 1, H2ac, H2AFL
计算分子量 130 aa, 14 kDa
观测分子量 17 kDa
GenBank蛋白编号BC017379
基因名称 HIST1H2AC
Gene ID (NCBI) 8334
RRIDAB_2878199
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93077
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIST1H2AC antibody 15953-1-APDownload protocol
IHC protocol for HIST1H2AC antibody 15953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biology (Basel)

Identification of NRAS Downstream Genes with CRISPR Activation Screening

Authors - Akiya Tatsumi
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