验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
18188-1-AP targets HIST1H1T in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag12908 Product name: Recombinant human HIST1H1T protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-206 aa of BC069517 Sequence: MSETVPAASASAGVAAMEKLPTKKRGRKPAGLISASRKVPNLSVSKLITEALSVSQERVGMSLVALKKALAAAGYDVEKNNSRIKLSLKSLVNKGILVQTRGTGASGSFKLSKKVIPKSTRSKAKKSVSAKTKKLVLSRDSKSPKTAKTNKRAKKPRATTPKTVRSGRKAKGAKGKQQQKSPVKARASKSKLTQHHEVNVRKATSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H1t |
| 别名 | Histone H1t, Histone H1, Histone, H1t, H1FT |
| 计算分子量 | 206 aa, 22 kDa |
| 观测分子量 | 22-30 kDa |
| GenBank蛋白编号 | BC069517 |
| 基因名称 | HIST1H1T |
| Gene ID (NCBI) | 3010 |
| RRID | AB_2878515 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P22492 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Testis-specific histone variants facilitate histone to protamine exchange during maturation of spermatozoa. Three testis-specific histone variants are regulated in male germ cells including H1T, H1T2 and HILS1 (encoded by H1ST1H1T, H1T2 and HILS1 respectively).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HIST1H1T antibody 18188-1-AP | Download protocol |
| IHC protocol for HIST1H1T antibody 18188-1-AP | Download protocol |
| WB protocol for HIST1H1T antibody 18188-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biol Reprod UHRF1 is indispensable for meiotic sex chromosome inactivation and interacts with the DNA damage response pathway in mice. | ||
Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control |
