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验证数据展示

  • WB analysis of MCF-7 using 19649-1-AP

    WB analysis of MCF-7 using 19649-1-AP

    WB result of Histone H1.2 antibody (19649-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H1.2 transfected MCF-7 cells.

  • IF Staining of HeLa using 19649-1-AP

    IF Staining of HeLa using 19649-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of L02 using 19649-1-AP

    WB analysis of L02 using 19649-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 19649-1-AP

    IHC staining of human ovary tumor using 19649-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19649-1-AP (HIST1H1C antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 19649-1-AP

    IF Staining of HepG2 using 19649-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of HeLa using 19649-1-AP

    IP experiment of HeLa using 19649-1-AP

    IP result of anti-Histone H1.2 (IP:19649-1-AP, 3ug; Detection:19649-1-AP 1:500) with HeLa cells lysate 1600ug.

  • IHC staining of human normal colon using 19649-1-AP

    IHC staining of human normal colon using 19649-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 19649-1-AP (Histone H1.2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

Histone H1.2 Polyclonal antibody

Histone H1.2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19649-1-AP

产品说明书

引用文献(35)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

Histone H1, histone cluster 1, H1c, HIST1H1C, H1F2, H1-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, human testis tissue, Jurkat cells, MCF-7 cells, A375 cells, mouse thymus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19649-1-AP targets Histone H1.2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, camelus bactrianus
免疫原

CatNo: Ag7503

Product name: Recombinant human Histone H1.2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-213 aa of BC002649

Sequence: MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKVKKAGGTKPKKPVGAAKKPKKAAGGATPKKSAKKTPKKAKKPAAATVTKKVAKSPKKAKVAKPKKAAKSAAKAVKPKAAKPKVVKPKKAAPKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 1, H1c
别名 Histone H1, histone cluster 1, H1c, HIST1H1C, H1F2, H1-2
计算分子量 21 kDa
观测分子量 32-33 kDa
GenBank蛋白编号BC002649
基因名称 Histone H1.2
Gene ID (NCBI) 3006
RRIDAB_10694432
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16403
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calculated molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa. (PMID: 18258596)

实验方案

Product Specific Protocols
IF protocol for Histone H1.2 antibody 19649-1-APDownload protocol
IHC protocol for Histone H1.2 antibody 19649-1-APDownload protocol
IP protocol for Histone H1.2 antibody 19649-1-APDownload protocol
WB protocol for Histone H1.2 antibody 19649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Mol Immunol

Mapping the holonomic signaling network that drives pathological changes in endotoxic shock.

Authors - Teng Teng
View Article
humanWB

Cancer Res

A20/TNFAIP3 Regulates the DNA Damage Response and Mediates Tumor Cell Resistance to DNA-Damaging Therapy.

Authors - Chuanzhen Yang
View Article
mouseWB

Nat Neurosci

Genome-wide distribution of linker histone H1.0 is independent of MeCP2.

Authors - Aya Ito-Ishida
View Article
humanChIP

Nat Commun

The nucleic acid binding protein SFPQ represses EBV lytic reactivation by promoting histone H1 expression

Authors - Laura A Murray-Nerger
View Article
mouseWB

Nat Commun

Histone functions as a cell-surface receptor for AGEs.

Authors - Masanori Itakura
View Article
humanWB

Mol Cell

CRAMP1 drives linker histone expression to enable Polycomb repression

Authors - Rachael E Matthews
View Article
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