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验证数据展示

  • WB analysis of HeLa using 14992-1-AP

    WB analysis of HeLa using 14992-1-AP

    WB result of HIRIP3 antibody (14992-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIRIP3 transfected HeLa cells.

  • IHC staining of human ovary using 14992-1-AP

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HIRIP3 antibody included
  • IHC staining of human ovary using 14992-1-AP

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HIRIP3 antibody included
  • IHC staining of human stomach cancer using 14992-1-AP

    IHC staining of human stomach cancer using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14992-1-AP (HIRIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIRIP3 antibody included
  • IHC staining of human stomach cancer using 14992-1-AP

    IHC staining of human stomach cancer using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14992-1-AP (HIRIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIRIP3 antibody included
  • IP experiment of HEK-293 using 14992-1-AP

    IP experiment of HEK-293 using 14992-1-AP

    IP result of anti-HIRIP3 (IP:14992-1-AP, 4ug; Detection:14992-1-AP 1:3000) with HEK-293 cells lysate 1600 ug.

  • Featured Product
  • KD/KO验证

HIRIP3 Polyclonal antibody

HIRIP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14992-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IP, ELISA

别名

HIRA-interacting protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human heart tissue, human skeletal muscle tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human stomach cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIRIP3 Ready-To-Use IHC Kit. HIRIP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14992-1-AP targets HIRIP3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原

CatNo: Ag6978

Product name: Recombinant human HIRIP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 207-556 aa of BC000588

Sequence: TAKKVEGNKGTKSLKESEQESEEEILAQKKEQREEEVEEEEKEEDEEKGDWKPRTRSNGRRKSAREERSCKQKSQAKRLLGDSDSEEEQKEAASSGDDSGRDREPPVQRKSEDRTQLKGGKRLSGSSEDEEDSGKGEPTAKGSRKMARLGSTSGEESDLEREVSDSEAGGGPQGERKNRSSKKSSRKGRTRSSSSSSDGSPEAKGGKAGSGRRGEDHPAVMRLKRYIRACGAHRNYKKLLGSCCSHKERLSILRAELEALGMKGTPSLGKCRALKEQREEAAEVASLDVANIISGSGRPRRRTAWNPLGEAAPPGELYRRTLDSDEERPRPAPPDWSHMRGIISSDGESN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HIRA interacting protein 3
别名 HIRA-interacting protein 3
计算分子量 62 kDa
观测分子量 69-90 kDa
GenBank蛋白编号BC000588
基因名称 HIRIP3
Gene ID (NCBI) 8479
RRIDAB_2117876
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BW71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.

实验方案

Product Specific Protocols
IHC protocol for HIRIP3 antibody 14992-1-APDownload protocol
IP protocol for HIRIP3 antibody 14992-1-APDownload protocol
WB protocol for HIRIP3 antibody 14992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Cell Neurosci

Integrative bioinformatics and machine learning identify iron metabolism genes MAP4, GPT, and HIRIP3 as diagnostic biomarkers and therapeutic targets in Alzheimer's disease

Authors - Xiaoqiong An
View Article
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