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验证数据展示

  • WB analysis of mouse skeletal muscle using 14992-1-AP

    WB analysis of mouse skeletal muscle using 14992-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14992-1-AP

    WB analysis of HeLa using 14992-1-AP

    WB result of HIRIP3 antibody (14992-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIRIP3 transfected HeLa cells.

  • IHC staining of human ovary using 14992-1-AP

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HIRIP3 antibody included
  • IHC staining of human ovary using 14992-1-AP

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HIRIP3 antibody included
  • IP experiment of HEK-293 using 14992-1-AP

    IP experiment of HEK-293 using 14992-1-AP

    IP result of anti-HIRIP3 (IP:14992-1-AP, 4ug; Detection:14992-1-AP 1:3000) with HEK-293 cells lysate 1600 ug.

  • Featured Product
  • KD/KO验证

HIRIP3 Polyclonal antibody

HIRIP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14992-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

HIRA-interacting protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, HeLa cells, human heart tissue, human skeletal muscle tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIRIP3 Ready-To-Use IHC Kit. HIRIP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14992-1-AP targets HIRIP3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6978

Product name: Recombinant human HIRIP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 207-556 aa of BC000588

Sequence: TAKKVEGNKGTKSLKESEQESEEEILAQKKEQREEEVEEEEKEEDEEKGDWKPRTRSNGRRKSAREERSCKQKSQAKRLLGDSDSEEEQKEAASSGDDSGRDREPPVQRKSEDRTQLKGGKRLSGSSEDEEDSGKGEPTAKGSRKMARLGSTSGEESDLEREVSDSEAGGGPQGERKNRSSKKSSRKGRTRSSSSSSDGSPEAKGGKAGSGRRGEDHPAVMRLKRYIRACGAHRNYKKLLGSCCSHKERLSILRAELEALGMKGTPSLGKCRALKEQREEAAEVASLDVANIISGSGRPRRRTAWNPLGEAAPPGELYRRTLDSDEERPRPAPPDWSHMRGIISSDGESN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HIRA interacting protein 3
别名 HIRA-interacting protein 3
计算分子量 62 kDa
观测分子量 69-90 kDa
GenBank蛋白编号BC000588
基因名称 HIRIP3
Gene ID (NCBI) 8479
RRIDAB_2117876
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BW71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.

实验方案

Product Specific Protocols
WB protocol for HIRIP3 antibody 14992-1-APDownload protocol
IHC protocol for HIRIP3 antibody 14992-1-APDownload protocol
IP protocol for HIRIP3 antibody 14992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols