验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse lung tissue, mouse heart tissue, human liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16814-1-AP targets HIP1R in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag10353 Product name: Recombinant human HIP1R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 266-615 aa of BC067085 Sequence: RASDMLYFKRLIQIPRLPEGPPNFLRASALAEHIKPVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPSGSVKDDRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQKALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKLKEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKEQLAFQVEQVKRESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLDTLSAEKDALSGAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQGRLAERVWPPQMQQHH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | huntingtin interacting protein 1 related |
| 别名 | HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655 |
| 计算分子量 | 615 aa, 70 kDa, 120 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC067085 |
| 基因名称 | HIP1R |
| Gene ID (NCBI) | 9026 |
| RRID | AB_2117572 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75146 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HIP1R antibody 16814-1-AP | Download protocol |
| IP protocol for HIP1R antibody 16814-1-AP | Download protocol |
| WB protocol for HIP1R antibody 16814-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.
| ||
Nat Cell Biol Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.
| ||
Nat Biomed Eng Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours. | ||
Nat Chem Biol HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.
| ||
Sci Adv The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.
| ||
J Clin Med HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis. |
