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HIP1-Specific Polyclonal antibody

HIP1-Specific Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 19688-1-AP

Print datasheet

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

验证数据展示

  • WB analysis of HEK-293 using 19688-1-AP

    WB analysis of HEK-293 using 19688-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19688-1-AP (HIP1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 19688-1-AP

    IHC staining of human prostate cancer using 19688-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human prostate cancer using 19688-1-AP

    IHC staining of human prostate cancer using 19688-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IP experiment of HEK-293 using 19688-1-AP

    IP experiment of HEK-293 using 19688-1-AP

    IP result of anti-HIP1-Specific (IP:19688-1-AP, 4ug; Detection:19688-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse colon tissue, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19688-1-AP targets HIP1-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name huntingtin interacting protein 1
Synonyms HIP 1, HIP I, HIP1, ILWEQ
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberNM_005338
Gene Symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_10640443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

实验方案

Product Specific Protocols
WB protocol for HIP1-Specific antibody 19688-1-APDownload protocol
IHC protocol for HIP1-Specific antibody 19688-1-APDownload protocol
IP protocol for HIP1-Specific antibody 19688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols