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验证数据展示

  • WB analysis using 22231-1-AP (same clone as 22231-1-PBS)

    WB analysis using 22231-1-AP (same clone as 22231-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 22231-1-AP (same clone as 22231-1-PBS)

    IP experiment of HeLa using 22231-1-AP (same clone as 22231-1-PBS)

    IP result of anti-HIP1 (IP:22231-1-AP, 4ug; Detection:22231-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 22231-1-AP (same clone as 22231-1-PBS)

    IF Staining of A549 using 22231-1-AP (same clone as 22231-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIP1 antibody (22231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

    IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

    IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

    IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

    IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIP1 antibody included
  • Featured Product
  • KD/KO Validated

HIP1 Polyclonal antibody, PBS Only

HIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 22231-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.

产品信息

22231-1-PBS targets HIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17576

Product name: Recombinant human HIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 435-764 aa of BC110545

Sequence: AELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 huntingtin interacting protein 1
别名
计算分子量 1037 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC110545
基因名称 HIP1
Gene ID (NCBI) 3092
RRIDAB_2879041
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO00291
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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