验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, rat brain tissue, rat thymus tissue, K-562 cells, Raji cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10717-1-AP targets HINT1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1142 Product name: Recombinant human HINT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-126 aa of BC007090 Sequence: MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histidine triad nucleotide binding protein 1 |
| 别名 | HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1 |
| 计算分子量 | 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC007090 |
| 基因名称 | HINT1 |
| Gene ID (NCBI) | 3094 |
| RRID | AB_2248272 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49773 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HINT1 antibody 10717-1-AP | Download protocol |
| IP protocol for HINT1 antibody 10717-1-AP | Download protocol |
| WB protocol for HINT1 antibody 10717-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells. | ||
Oncogene Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells. | ||
J Med Chem A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide | ||
Cell Cycle The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells. | ||
Pharmacogenomics J Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence. | ||
