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验证数据展示

  • IF Staining of HeLa using 10066-2-AP

    IF Staining of HeLa using 10066-2-AP

    Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • WB analysis of human liver using 10066-2-AP

    WB analysis of human liver using 10066-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 10066-2-AP

    WB analysis using 10066-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10066-2-AP

    WB analysis using 10066-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 10066-2-AP

    IP experiment of L02 using 10066-2-AP

    IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug.

  • IP experiment of L02 using 10066-2-AP

    IP experiment of L02 using 10066-2-AP

    IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug.

  • Featured Product
  • KD/KO验证

HINFP Polyclonal antibody

HINFP Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 10066-2-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (2)

别名

MIZF, Methyl-CpG-binding protein 2-interacting zinc finger protein, MBD2-interacting zinc finger protein, Histone nuclear factor P, HiNF-P

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue, L02 cells
Positive IP detected inL02 cells
Positive IF/ICC detected inHela cells, L02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10066-2-AP targets HINFP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 HINFP fusion protein Ag0115 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone H4 transcription factor
别名 MIZF, Methyl-CpG-binding protein 2-interacting zinc finger protein, MBD2-interacting zinc finger protein, Histone nuclear factor P, HiNF-P
计算分子量 60 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC001073
基因名称 HINFP
Gene ID (NCBI) 25988
RRIDAB_2117412
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQA5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.

实验方案

Product Specific Protocols
WB protocol for HINFP antibody 10066-2-APDownload protocol
IF protocol for HINFP antibody 10066-2-APDownload protocol
IP protocol for HINFP antibody 10066-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Oncogene

Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.

Authors - Xianchong Zheng
  • KO Validated
View Article