验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue, L02 cells |
| Positive IP detected in | L02 cells |
| Positive IF/ICC detected in | Hela cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10066-2-AP targets HINFP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0115 Product name: Recombinant human HINFP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 215-452 aa of BC001073 Sequence: LDHIRRQTSLDQQHFQCSHCSKRFATERLLRDHMRNHVNHYKCPLCDMTCPLPSSLRNHMRFRHSEDRPFKCDCCDYSCKNLIDLQKHLDTHSEEPAYRCDFENCTFSARSLCSIKSHYRKVHEGDSEPRYKCHVCDKCFTRGNNLTVHLRKKHQFKWPSGHPRFRYKEHEDGYMRLQLVRYESVELTQQLLRQPQEGSGLGTSLNESSLQGIILETVPGEPGRKEEEEEGKGSEGTA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histone H4 transcription factor |
| 别名 | MIZF, Methyl-CpG-binding protein 2-interacting zinc finger protein, MBD2-interacting zinc finger protein, Histone nuclear factor P, HiNF-P |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC001073 |
| 基因名称 | HINFP |
| Gene ID (NCBI) | 25988 |
| RRID | AB_2117412 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQA5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HINFP antibody 10066-2-AP | Download protocol |
| IP protocol for HINFP antibody 10066-2-AP | Download protocol |
| WB protocol for HINFP antibody 10066-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
