验证数据展示

WB analysis of A431 using 27650-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 27650-1-AP
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 27650-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 27650-1-AP
Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using HIF3A antibody (27650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO验证

HIF3A Polyclonal antibody

HIF3A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 27650-1-AP

产品说明书

引用文献(7)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

HIF3 alpha, HIF 3A4, HIF 3A2, HIF 3A, HIF 3 alpha

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (7)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	Cobalt Chloride treated HeLa cells, A431 cells
Positive IF/ICC detected in	Tunicamycin treated HeLa cells

Planning an IHC experiment? We recommend our IHCeasy HIF3A Ready-To-Use IHC Kit. HIF3A primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 3 publications below

产品信息

27650-1-AP targets HIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IF/ICC, ELISA Application Description
文献引用应用	WB, IHC
经测试反应性	human
文献引用反应性	human, mouse
免疫原	CatNo: Ag26574 Product name: Recombinant human HIF3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 401-486 aa of NM_022462 Sequence: MALAADPRRFCSPDLRRLLGPILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDADALD 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	hypoxia inducible factor 3, alpha subunit
别名	HIF3 alpha, HIF 3A4, HIF 3A2, HIF 3A, HIF 3 alpha
计算分子量	72 kDa
观测分子量	72 kDa
GenBank蛋白编号	NM_022462
基因名称	HIF3A
Gene ID (NCBI)	64344
RRID	AB_2880939
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9Y2N7
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

HIF3A, Hypoxia-inducible factor 3-alpha, acts as a transcriptional regulator in adaptive response to low oxygen tension (PMID:11573933, PMID:16126907, PMID:19694616, PMID:20416395, PMID:21069422). It functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. HIF3A also plays a role in the development of the cardiorespiratory system. HIF3A was up-regulated by hypoxia (PMID:16775626). Multiple alternatively spliced transcript variants have been found.

实验方案

Product Specific Protocols
IF protocol for HIF3A antibody 27650-1-AP	Download protocol
WB protocol for HIF3A antibody 27650-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nat Commun Identification of oleoylethanolamide as an endogenous ligand for HIF-3α. Authors - Xiaotong Diao View Article
human	WB	Mol Metab Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort. Authors - Agné Kulyté KD Validated View Article
mouse	WB	Front Cell Dev Biol HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring. Authors - Francesca Cuomo KD Validated View Article
mouse	WB	Toxicol Lett Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis. Authors - Wenchao Gu View Article
human	IHC	ERJ Open Res Dynamic analysis of gene signatures in the progression of COPD Authors - Junchao Jiang View Article
mouse	IHC	Bioact Mater Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform Authors - Dong Guo View Article

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验证数据展示

WB analysis of A431 using 27650-1-AP

WB analysis using 27650-1-AP

WB analysis using 27650-1-AP

IF Staining of HeLa using 27650-1-AP

HIF3A Polyclonal antibody

HIF3A Polyclonal Antibody for WB, IF/ICC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Identification of oleoylethanolamide as an endogenous ligand for HIF-3α.

Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort.

HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.

Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis.

Dynamic analysis of gene signatures in the progression of COPD

Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform