- Featured Product
- KD/KO Validated
HIF3A Polyclonal antibody
HIF3A Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
IF, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Cobalt Chloride treated HeLa cells, A431 cells |
| Positive IF detected in | Tunicamycin treated HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HIF3A Ready-To-Use IHC Kit. HIF3A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
27650-1-AP targets HIF3A in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IF, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | HIF3A fusion protein Ag26574 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hypoxia inducible factor 3, alpha subunit |
| Synonyms | bHLHe17, HIF 3 alpha, HIF 3A, HIF 3A2, HIF 3A4, HIF3 alpha, HIF3 alpha 1, HIF3A, Inhibitory PAS domain protein, IPAS, Member of PAS protein 7, MOP7, PASD7 |
| Calculated Molecular Weight | 72 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | NM_022462 |
| Gene Symbol | HIF3A |
| Gene ID (NCBI) | 64344 |
| RRID | AB_2880939 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF3A, Hypoxia-inducible factor 3-alpha, acts as a transcriptional regulator in adaptive response to low oxygen tension (PMID:11573933, PMID:16126907, PMID:19694616, PMID:20416395, PMID:21069422). It functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. HIF3A also plays a role in the development of the cardiorespiratory system. HIF3A was up-regulated by hypoxia (PMID:16775626). Multiple alternatively spliced transcript variants have been found.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HIF3A antibody 27650-1-AP | Download protocol |
| IF protocol for HIF3A antibody 27650-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Metab Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort.
| ||
Front Cell Dev Biol HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.
| ||
Toxicol Lett Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis. | ||