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  • Featured Product
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HIF1AN Polyclonal antibody

HIF1AN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10646-1-AP

Print datasheet

Synonyms

Factor inhibiting HIF 1, FIH 1, FIH1, HIF1AN

验证数据展示

  • WB analysis of HEK-293 using 10646-1-AP

    WB analysis of HEK-293 using 10646-1-AP

    WB result of HIF1AN antibody (10646-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF1AN transfected HEK-293 cells.

  • WB analysis using 10646-1-AP

    WB analysis using 10646-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10646-1-AP (HIF1AN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 10646-1-AP

    IHC staining of human lung cancer using 10646-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10646-1-AP (HIF1AN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIF1AN antibody included
  • IHC staining of human lung cancer using 10646-1-AP

    IHC staining of human lung cancer using 10646-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10646-1-AP (HIF1AN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIF1AN antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIF1AN Ready-To-Use IHC Kit. HIF1AN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10646-1-AP targets HIF1AN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen HIF1AN fusion protein Ag0997 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypoxia inducible factor 1, alpha subunit inhibitor
Synonyms Factor inhibiting HIF 1, FIH 1, FIH1, HIF1AN
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC007719
Gene Symbol HIF1AN
Gene ID (NCBI) 55662
RRIDAB_2232963
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF1AN (Hypoxia-inducible factor 1-alpha inhibitor) is also named FIH1. It is identified as a molecular oxygen-dependent dioxygenase that blunts the transcriptional activity of HIF and has also been implicated in HIF-dependent and -independent hypoxia responses(PMID:20720525). Structural studies indicate that an FIH-1 homodimer forms in solution and is essential for FIH-1 activity(PMID:16154531).

实验方案

Product Specific Protocols
WB protocol for HIF1AN antibody 10646-1-APDownload protocol
IHC protocol for HIF1AN antibody 10646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Bioact Mater

Development of a novel RNAi therapy: Engineered miR-31 exosomes promoted the healing of diabetic wounds.

Authors - Jinghuan Huang
View Article
humanWB

Am J Cancer Res

Experimental and clinical evidence suggests that GRPEL2 plays an oncogenic role in HCC development.

Authors - Ming-Chun Lai
View Article