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验证数据展示

  • WB analysis using 20960-1-AP (same clone as 20960-1-PBS)

    WB analysis using 20960-1-AP (same clone as 20960-1-PBS)

    Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

    WB analysis of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

    WB result of HIF-1 alpha antibody (20960-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells. This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 20960-1-AP (same clone as 20960-1-PBS)

    IHC staining of human thyroid cancer using 20960-1-AP (same clone as 20960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 20960-1-AP (same clone as 20960-1-PBS)

    IHC staining of human thyroid cancer using 20960-1-AP (same clone as 20960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

    IF Staining of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (20960-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

    IP experiment of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

    IP result of anti-HIF-1 alpha (IP:20960-1-AP, 4ug; Detection:20960-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HIF-1 alpha Polyclonal antibody, PBS Only

HIF-1 alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20960-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

20960-1-PBS targets HIF-1 alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 HIF-1 alpha fusion protein Ag15198 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
别名 HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1
计算分子量 826 aa, 93 kDa
观测分子量 120 kDa
GenBank蛋白编号BC012527
基因名称 HIF1A
Gene ID (NCBI) 3091
RRIDAB_10732601
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16665
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .