验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MG-63 cells, U2OS cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33973-1-AP targets HICE1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40571 Product name: Recombinant human HICE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-284 aa of BC010176 Sequence: DVGDSEENVQVLDLLSELKDVTAKKDLELRRSFAQVLELSAEASKEAALANQEVWEETQGMAPPSRWYFNQDSACRESGGAPKNTPLSEDDNPGASSAPAQATFISPSEDFSSSSQAEVPPSLSRSGRDLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HEC1/NDC80 interacting, centrosome associated 1 |
| 别名 | HAUS augmin-like complex subunit 8, HAUS8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48 |
| 计算分子量 | 410 aa, 45 kDa |
| 观测分子量 | 38kDa, 45kDa |
| GenBank蛋白编号 | BC010176 |
| 基因名称 | HICE1 |
| Gene ID (NCBI) | 93323 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9BT25 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HICE1 antibody 33973-1-AP | Download protocol |
| WB protocol for HICE1 antibody 33973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
