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验证数据展示

  • WB analysis using 13466-1-AP

    WB analysis using 13466-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13466-1-AP (HIBADH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13466-1-AP

    IHC staining of human liver cancer using 13466-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13466-1-AP (HIBADH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 13466-1-AP

    IHC staining of human liver cancer using 13466-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13466-1-AP (HIBADH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse heart using 13466-1-AP

    IP experiment of mouse heart using 13466-1-AP

    IP result of anti-HIBADH (IP:13466-1-AP, 4ug; Detection:13466-1-AP 1:800) with mouse heart tissue lysate 4000ug.

  • Featured Product
  • KD/KO验证

HIBADH Polyclonal antibody

HIBADH Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13466-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

HIBADH, NS5ATP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse ovary tissue, mouse thymus tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13466-1-AP targets HIBADH in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 HIBADH fusion protein Ag4258 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 3-hydroxyisobutyrate dehydrogenase
别名 HIBADH, NS5ATP1
计算分子量 336 aa, 35 kDa
观测分子量 32-35 kDa
GenBank蛋白编号BC032324
基因名称 HIBADH
Gene ID (NCBI) 11112
RRIDAB_2116838
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31937
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIBADH antibody 13466-1-APDownload protocol
IHC protocol for HIBADH antibody 13466-1-APDownload protocol
IP protocol for HIBADH antibody 13466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Med

PMID: 26950361

Authors - Cholsoon Jang
  • KD Validated
View Article
mouseWB

PLoS One

Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.

Authors - Yuan Wang
View Article