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  • Featured Product
  • KD/KO Validated

HIBADH Polyclonal antibody

HIBADH Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13466-1-AP

Print datasheet

Synonyms

HIBADH, NS5ATP1

验证数据展示

  • WB analysis using 13466-1-AP

    WB analysis using 13466-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13466-1-AP (HIBADH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13466-1-AP

    IHC staining of human liver cancer using 13466-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13466-1-AP (HIBADH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 13466-1-AP

    IHC staining of human liver cancer using 13466-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13466-1-AP (HIBADH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse heart using 13466-1-AP

    IP experiment of mouse heart using 13466-1-AP

    IP result of anti-HIBADH (IP:13466-1-AP, 4ug; Detection:13466-1-AP 1:800) with mouse heart tissue lysate 4000ug.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse ovary tissue, mouse thymus tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13466-1-AP targets HIBADH in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen HIBADH fusion protein Ag4258 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 3-hydroxyisobutyrate dehydrogenase
Synonyms HIBADH, NS5ATP1
Calculated Molecular Weight 336 aa, 35 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC032324
Gene Symbol HIBADH
Gene ID (NCBI) 11112
RRIDAB_2116838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31937
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIBADH antibody 13466-1-APDownload protocol
IHC protocol for HIBADH antibody 13466-1-APDownload protocol
IP protocol for HIBADH antibody 13466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Med

PMID: 26950361

Authors - Cholsoon Jang
  • KD Validated
View Article
mouseWB

PLoS One

Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.

Authors - Yuan Wang
View Article