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验证数据展示

  • WB analysis using 29466-1-AP (same clone as 29466-1-PBS)

    WB analysis using 29466-1-AP (same clone as 29466-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29466-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 29466-1-AP (same clone as 29466-1-PBS)

    IHC staining of mouse pancreas using 29466-1-AP (same clone as 29466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 29466-1-AP (same clone as 29466-1-PBS)

    IHC staining of mouse testis using 29466-1-AP (same clone as 29466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 29466-1-AP (same clone as 29466-1-PBS)

    IHC staining of mouse testis using 29466-1-AP (same clone as 29466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 29466-1-AP (same clone as 29466-1-PBS)

    IHC staining of mouse brain using 29466-1-AP (same clone as 29466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 29466-1-AP (same clone as 29466-1-PBS)

    IF Staining of HeLa using 29466-1-AP (same clone as 29466-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HHIP antibody (29466-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 29466-1-AP (same clone as 29466-1-PBS)

    IHC staining of mouse pancreas using 29466-1-AP (same clone as 29466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

HHIP Polyclonal antibody, PBS Only

HHIP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29466-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29466-1-PBS targets HHIP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30869

Product name: Recombinant human HHIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 18-277 aa of BC025311

Sequence: FFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hedgehog interacting protein
别名
计算分子量 700 aa, 79 kDa
观测分子量85 kDa
GenBank蛋白编号BC025311
基因名称 HHIP
Gene ID (NCBI) 64399
RRIDAB_3086136
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QV1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. HHIP is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH.

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