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验证数据展示

  • WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

    WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26881-1-AP (HGF alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

    WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26881-1-AP (HGF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    IHC staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26881-1-AP (HGF alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HGF antibody included
  • IHC staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    IHC staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26881-1-AP (HGF alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HGF antibody included
  • IF Staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    IF Staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using HGF antibody (26881-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    IF Staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using HGF antibody (26881-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 26881-1-AP (same clone as 26881-1-PBS)

    IF Staining of HepG2 using 26881-1-AP (same clone as 26881-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGF Alpha antibody (26881-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

HGF Polyclonal antibody, PBS Only

HGF Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 26881-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

HGF Alpha, Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HGF Ready-To-Use IHC Kit. HGF primary antibody included.

产品信息

26881-1-PBS targets HGF in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25456

Product name: Recombinant human HGF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 118-157 aa of BC130286

Sequence: LYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hepatocyte growth factor (hepapoietin A; scatter factor)
别名 HGF Alpha, Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A
观测分子量 69 kDa
GenBank蛋白编号BC130286
基因名称 HGF
Gene ID (NCBI) 3082
RRIDAB_2880668
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14210
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa α-subunit (containing four kringle domains) and the 34 kDa β-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.

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