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验证数据展示

  • WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

    WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 84931-5-RR (same clone as 84931-5-PBS)

    IHC staining of rat liver using 84931-5-RR (same clone as 84931-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84931-5-RR (HGD antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84931-5-RR

    Affinity and Kinetic Characterization of 84931-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84931-5-RR against Human HGD were performed. The affinity constant is 1.38 nM.

HGD Recombinant monoclonal antibody, PBS Only

HGD Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84931-5-PBS

产品说明书

CloneNo. 242485E11

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

242485E11, AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84931-5-PBS targets HGD in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag9544

Product name: Recombinant human HGD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-329 aa of BC020792

Sequence: MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLYRILPSVSHKPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLCGAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEICVIQRGMRFSIDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTVLTALRRPARSSWHLRGLPMAPWHLCLNHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 homogentisate 1,2-dioxygenase (homogentisate oxidase)
别名 242485E11, AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase
计算分子量 37 kDa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC020792
基因名称 HGD
Gene ID (NCBI) 3081
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ93099
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

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