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验证数据展示

  • WB analysis using 16465-1-AP

    WB analysis using 16465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 16465-1-AP

    IHC staining of mouse liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16465-1-AP (HGD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HGD Polyclonal antibody

HGD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16465-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase, Homogentisate 1,2-dioxygenase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, rat liver tissue, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16465-1-AP targets HGD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9544

Product name: Recombinant human HGD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-329 aa of BC020792

Sequence: MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLYRILPSVSHKPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLCGAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEICVIQRGMRFSIDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTVLTALRRPARSSWHLRGLPMAPWHLCLNHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homogentisate 1,2-dioxygenase (homogentisate oxidase)
别名 AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase, Homogentisate 1,2-dioxygenase
计算分子量 37 kDa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC020792
基因名称 HGD
Gene ID (NCBI) 3081
RRIDAB_2248397
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93099
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

实验方案

Product Specific Protocols
WB protocol for HGD antibody 16465-1-APDownload protocol
IHC protocol for HGD antibody 16465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols