验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, MCF-7 cells, MDA-MB-231 cells, human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33841-1-AP targets HFM1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40875 Product name: Recombinant human HFM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 333-518 aa of BC130525 Sequence: LPKYELKVEQITRYSDTTAEILVTVILRNFEQLQTKRTASDSHYVTLIIGDADNQVVYLHKITDSVLLKAGSWAKKIAVKRALKSEDLSINLISSEFVGLDIQQKLTVFYLEPKRFGNQITMQRKSETQISHSKHSDISTIAGPNKGTTASKKPGNRECNHLCKSKHTCGHDCCKIGVAQKSEIKE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) |
| 别名 | SEC3D1 |
| 观测分子量 | 63-70 kDa |
| GenBank蛋白编号 | BC130525 |
| 基因名称 | HFM1 |
| Gene ID (NCBI) | 164045 |
| RRID | AB_3743089 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | A2PYH4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HFM1 (helicase for meiosis 1), also known as MER3, POF9, Si-11, SEC63D1, and Si-11-6, is a candidate gene of POI. HFM1 was reported to be mainly expressed in germline cells, which is required for crossover formation and complete synapsis of homologous chromosomes in spermiogenesis HFM1, expressed specifically in germline tissues, is a typical ATP-dependent DNA helicase mediating meiosis (PMID: 39725823, 37020390).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HFM1 antibody 33841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
