×
Use Able AI chat for product recommendations

验证数据展示

  • IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83501-7-RR (same clone as 83501-7-PBS)

    FC experiment of HEK-293 using 83501-7-RR (same clone as 83501-7-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    FC experiment of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83501-7-RR

    Affinity and Kinetic Characterization of 83501-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83501-7-RR against Human HEY2 were performed. The affinity constant is 1.08 nM.

HEY2 Recombinant monoclonal antibody, PBS Only

HEY2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83501-7-PBS

产品说明书

CloneNo. 240474F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

240474F2, bHLHb32, Cardiovascular helix-loop-helix factor 1, CHF1, Class B basic helix-loop-helix protein 32

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

83501-7-PBS targets HEY2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30988

Product name: Recombinant human HEY2 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-337 aa of BC007707

Sequence: MKRPCEETTSESDMDETIDVGSENNYSGQSTSSVIRLNSPTTTSQIMARKKRRGIIEKRRRDRINNSLSELRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDAHALAMDFMSIGFRECLTEVARYLSSVEGLDSSDPLRVRLVSHLSTCATQREAAAMTSSMAHHHHPLHPHHWAAAFHHLPAALLQPNGLHASESTPCRLSTTSEVPPAHGSALLTATFAHADSALRMPSTGSVAPCVPPLSTSLLSLSATVHAAAAAATAAAHSFPLSFAGAFPMLPPNAAAAVAAATAISPPLSVSATSSPQQTSSGTNNKPYRPWGTEVGAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hairy/enhancer-of-split related with YRPW motif 2
别名 240474F2, bHLHb32, Cardiovascular helix-loop-helix factor 1, CHF1, Class B basic helix-loop-helix protein 32
计算分子量 36 kDa
GenBank蛋白编号BC007707
基因名称 HEY2
Gene ID (NCBI) 23493
RRIDAB_3671127
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBP5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system.

||
New chat

Able™

正在加载，请稍候...