验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells |
| Positive IP detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10597-1-AP targets HEY2 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, chIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | HEY2 fusion protein Ag0913 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hairy/enhancer-of-split related with YRPW motif 2 |
| 别名 | GRL, GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 33-40 kDa |
| GenBank蛋白编号 | BC007707 |
| 基因名称 | HEY2 |
| Gene ID (NCBI) | 23493 |
| RRID | AB_2118415 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBP5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HEY2 antibody 10597-1-AP | Download protocol |
| IF protocol for HEY2 antibody 10597-1-AP | Download protocol |
| IP protocol for HEY2 antibody 10597-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. | ||
Nat Neurosci Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination. | ||
Nat Commun Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis. | ||
Nat Commun Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells. | ||
Nat Commun Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia. |
