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验证数据展示

  • WB analysis using 16229-1-AP (same clone as 16229-1-PBS)

    WB analysis using 16229-1-AP (same clone as 16229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16229-1-AP (HEXB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 16229-1-AP (same clone as 16229-1-PBS)

    IHC staining of human kidney using 16229-1-AP (same clone as 16229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16229-1-AP (HEXB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HEXB antibody included
  • IHC staining of human kidney using 16229-1-AP (same clone as 16229-1-PBS)

    IHC staining of human kidney using 16229-1-AP (same clone as 16229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16229-1-AP (HEXB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HEXB antibody included
  • Featured Product
  • KD/KO Validated

HEXB Polyclonal antibody, PBS Only

HEXB Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16229-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HEXB Ready-To-Use IHC Kit. HEXB primary antibody included.

产品信息

16229-1-PBS targets HEXB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8683

Product name: Recombinant human HEXB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-332 aa of BC017378

Sequence: MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hexosaminidase B (beta polypeptide)
别名
计算分子量 556 aa, 63 kDa
观测分子量 63-67 kDa
GenBank蛋白编号BC017378
基因名称 HEXB
Gene ID (NCBI) 3074
RRIDAB_2264075
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07686
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The HEXB (Beta-hexosaminidase subunit beta) gene encodes the beta subunit of the enzyme hexosaminidase, which is involved in the breakdown of gangliosides. It is responsible for the degradation of GM2 gangliosides, and has a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Refer to the domains of HEXB and glycosylation, there will be some different bands with MW 63-67 kDa precursor; 59-63 kDa signal peptide removed; 50-55 kDa subunit beta (or signal and propeptide removed); 20-25 kDa subunit beta chain B; ~30 kDa subunit beta chain A (Uniprot).

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