验证数据展示
经过测试的应用
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17927-1-AP targets HESX1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | HESX1 fusion protein Ag12348 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | HESX homeobox 1 |
别名 | ANF, hAnf, HESX homeobox 1, HESX1, Homeobox protein ANF, RPX |
计算分子量 | 185 aa, 21 kDa |
GenBank蛋白编号 | BC093979 |
基因名称 | HESX1 |
Gene ID (NCBI) | 8820 |
RRID | AB_2118120 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UBX0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HESX1 (Homeobox gene expressed in stem cells 1) is a homeobox transcriptional repressor that was initially isolated from murine embryonic stem cells (ESCs). Hesx1 is crucial for proper forebrain and pituitary development in both mice and humans, and inactivating mutations in HESX1 underlie septo-optic dysplasia.
实验方案
Product Specific Protocols | |
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IHC protocol for HESX1 antibody 17927-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |