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HERC5 Polyclonal antibody

HERC5 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 22692-1-AP

Print datasheet

Synonyms

CEB1, CEBP1, Cyclin E binding protein 1, E3 ISG15 protein ligase HERC5, hect domain and RLD 5, HERC5

验证数据展示

  • IP experiment of HEK-293 using 22692-1-AP

    IP experiment of HEK-293 using 22692-1-AP

    IP result of anti-HERC5 (IP:22692-1-AP, 4ug; Detection:22692-1-AP 1:300) with HEK-293 cells lysate 2800ug.

  • IHC staining of mouse testis using 22692-1-AP

    IHC staining of mouse testis using 22692-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22692-1-AP (HERC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 22692-1-AP

    IHC staining of mouse testis using 22692-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22692-1-AP (HERC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22692-1-AP is HERC5 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen HERC5 fusion protein Ag18449 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hect domain and RLD 5
Synonyms CEB1, CEBP1, Cyclin E binding protein 1, E3 ISG15 protein ligase HERC5, hect domain and RLD 5, HERC5
Calculated Molecular Weight 1024 aa, 117 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC140716
Gene Symbol HERC5
Gene ID (NCBI) 51191
RRIDAB_2879151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9UII4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for HERC5 antibody 22692-1-APDownload protocol
IP protocol for HERC5 antibody 22692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

MBio

ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.

Authors - Edmund Osei Kuffour
View Article
humanWB

Int J Mol Med

ISG15 inhibits cancer cell growth and promotes apoptosis.

Authors - Mei-Juan Zhou
View Article
human,mouseWB,CoIP

J Clin Invest

Posttranslational ISGylation of NLRP3 by HERCs enzymes facilitates inflammasome activation in models of inflammation

Authors - Ying Qin
  • KD Validated
View Article
humanWB

Cell Rep

Regulation of STING activity in DNA sensing by ISG15 modification

Authors - Chaohui Lin
View Article