• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IP experiment of HEK-293 using 22692-1-AP

    IP experiment of HEK-293 using 22692-1-AP

    IP result of anti-HERC5 (IP:22692-1-AP, 4ug; Detection:22692-1-AP 1:300) with HEK-293 cells lysate 2800ug.

HERC5 Polyclonal antibody

HERC5 Polyclonal Antibody for IP, ELISA
Cat No. 22692-1-AP

产品说明书

引用文献(9)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IP, ELISA and More (2)

别名

E3 ISG15--protein ligase HERC5, E3 ISG15 protein ligase HERC5, Cyclin-E-binding protein 1, Cyclin E binding protein 1, CEBP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22692-1-AP targets HERC5 in IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IP, ELISA Application Description
文献引用应用IHC, IP, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag18449

Product name: Recombinant human HERC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-342 aa of BC140716

Sequence: MERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHRALLRRVEVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIHSVDQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hect domain and RLD 5
别名 E3 ISG15--protein ligase HERC5, E3 ISG15 protein ligase HERC5, Cyclin-E-binding protein 1, Cyclin E binding protein 1, CEBP1
计算分子量 1024 aa, 117 kDa
观测分子量 117 kDa
GenBank蛋白编号BC140716
基因名称 HERC5
Gene ID (NCBI) 51191
RRIDAB_2879151
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9UII4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IP protocol for HERC5 antibody 22692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

MBio

ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.

Authors - Edmund Osei Kuffour
View Article
humanWB

Int J Mol Med

ISG15 inhibits cancer cell growth and promotes apoptosis.

Authors - Mei-Juan Zhou
View Article
human,mouseWB,CoIP

J Clin Invest

Posttranslational ISGylation of NLRP3 by HERCs enzymes facilitates inflammasome activation in models of inflammation

Authors - Ying Qin
  • KD Validated
View Article
humanWB

Cell Rep

Regulation of STING activity in DNA sensing by ISG15 modification

Authors - Chaohui Lin
View Article
human,mouseCoIP

Cell Rep

ISGylation by HERCs facilitates STING activation

Authors - Ying Qin
View Article
human

Adv Sci (Weinh)

Autophagy Deficiency Induced by SAT1 Potentiates Tumor Progression in Triple-Negative Breast Cancer

Authors - Wenwen Tian
View Article