验证数据展示
经过测试的应用
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
11148-1-AP targets Hephaestin in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 | Hephaestin fusion protein Ag1604 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hephaestin |
| 别名 | CPL, HEPH, Hephaestin, KIAA0698 |
| 计算分子量 | 130 kDa |
| GenBank蛋白编号 | BC011561 |
| 基因名称 | Hephaestin |
| Gene ID (NCBI) | 9843 |
| RRID | AB_11064728 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQS7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hephaestin, also named as KIAA0698, belongs to the multicopper oxidase family. It is provied as ferroxidase of cellular iron export. HEPH is implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. HEPE is active in placenta but may not play a key role in placental iron transport.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Hephaestin antibody 11148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biochem Funct Ferroportin 1 and hephaestin expression in BeWo cell line with different iron treatment. | ||
Chin Herb Med Neuroprotective effects of kukoamine A on 6-OHDA-induced Parkinson's model through apoptosis and iron accumulation inhibition |