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验证数据展示

  • WB analysis of mouse brain using 18177-1-AP (same clone as 18177-1-PBS)

    WB analysis of mouse brain using 18177-1-AP (same clone as 18177-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

  • WB analysis using 18177-1-AP (same clone as 18177-1-PBS)

    WB analysis using 18177-1-AP (same clone as 18177-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 18177-1-AP (same clone as 18177-1-PBS)

    IHC staining of mouse brain using 18177-1-AP (same clone as 18177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18177-1-AP (HEPACAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HEPACAM antibody included
  • IHC staining of rat brain using 18177-1-AP (same clone as 18177-1-PBS)

    IHC staining of rat brain using 18177-1-AP (same clone as 18177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18177-1-AP (HEPACAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HEPACAM antibody included
  • IHC staining of human liver using 18177-1-AP (same clone as 18177-1-PBS)

    IHC staining of human liver using 18177-1-AP (same clone as 18177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18177-1-AP (HEPACAM Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HEPACAM antibody included
  • IHC staining of human liver using 18177-1-AP (same clone as 18177-1-PBS)

    IHC staining of human liver using 18177-1-AP (same clone as 18177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18177-1-AP (HEPACAM Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HEPACAM antibody included
  • IF Staining of MCF-7 using 18177-1-AP (same clone as 18177-1-PBS)

    IF Staining of MCF-7 using 18177-1-AP (same clone as 18177-1-PBS)

    mmunofluorescent analysis of MCF-7 cells, using HEPACAM antibody 18177-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 18177-1-AP (same clone as 18177-1-PBS)

    IP experiment of rat brain using 18177-1-AP (same clone as 18177-1-PBS)

    IP result of anti-HEPACAM (IP:18177-1-AP, 4ug; Detection:18177-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 18177-1-PBS in a different storage buffer formulation.

HEPACAM Polyclonal antibody, PBS Only

HEPACAM Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18177-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

glialCAM, Hepatic and glial cell adhesion molecule, Protein hepaCAM

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HEPACAM Ready-To-Use IHC Kit. HEPACAM primary antibody included.

产品信息

18177-1-PBS targets HEPACAM in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12870

Product name: Recombinant human HEPACAM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-242 aa of BC104831

Sequence: ITSPVRLIHGTVGKSALLSVQYSSTSSDRPVVKWQLKRDKPVTVVQSIGTEVIGTLRPDYRDRIRLFENGSLLLSDLQLADEGTYEVEISITDDTFTGEKTINLTVDVPISRPQVLVASTTVLELSEAFTLNCSHENGTKPSYTWLKDGKPLLNDSRMLLSPDQKVLTITRVLMEDDDLYSCVVENPISQGRSLPVKITVYRRSSLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hepatocyte cell adhesion molecule
别名 glialCAM, Hepatic and glial cell adhesion molecule, Protein hepaCAM
计算分子量 416 aa, 46 kDa
观测分子量 46-72 kDa
GenBank蛋白编号BC104831
基因名称 HEPACAM
Gene ID (NCBI) 220296
RRIDAB_2279680
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14CZ8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HepaCAM (hepatocyte cell adhesion molecule), also known as GlialCAM, is a single-pass type I membrane glycoprotein of 416 amino acids. It displays a typical structure of immunoglobulin (Ig)-like adhesion molecules including two extracellular Ig-like domains, a transmembrane segment, and a cytoplasmic tail. It has been shown that hepaCAM forms a cis-homodimer on the cell surface, and modulates cell-matrix interaction. It is predominantly expressed in the CNS glial cells. Defects in HEPACAM gene are the cause of megalencephalic leukoencephalopathy with subcortical cysts type 2A (MLC2A). HEPACAM has also been suggested as a tumor suppressor gene.

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